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Inspire - Model HILIC (New) -Normal Phase Chromatography of Polar and Ionic Compounds
HILIC is normal phase chromatography of polar and ionic compounds under RP conditions. It combines the characteristics of three methods in LC – RP, NP, and IC. Inspire HILIC columns retain a water-enriched layer on the surface of the silica. This water layer facilitates the transfer of polar compounds onto the stationary phase for increased retention. Separation is achieved through the partitioning of polar solutes from the high concentration, water-miscible, organic mobile phase into the hydrophilic surface environment. Polar solutes exhibit increased retention, and elute in the order of increasing hydrophilicity. HILIC is ideal for separating and retaining polar compounds that may not retain on traditional RP packings. Unlike reversed-phase columns, Inspire HILIC columns retain highly polar compounds with only small amounts of water in the mobile phase. These more volatile mobile phases increase sensitivity with microbore applications.
- Unique selectivity and increased retention for highly polar compounds
- Increased sensitivity and lower detection limits with microbore
- Enhanced retention for hydrophilic/polar compounds
- Increased laboratory throughput and productivity
- Superior batch-to-batch reproducibility
- Suitable for the separation of hydrophilic compounds such as polar acids and bases, polar compounds, nucleosides, oligonucleotides, amino acids, peptides, and water-soluble vitamins
Antivirals
- Column: Inspire Hilic, 150 × 4.6 mm, 5 µm
- Cat. No.: 81401
- Mobile Phase: MeCN:10 mM ammonium formate (pH 3.0) = 80:20
- Flow Rate: 1.0 mL/min
- Detection: Ambient
- Temperature: UV 254 nm
- Sample:
- Acyclovir
- Ganciclovir
- Column: Inspire C18, 150 × 4.6 mm, 5 µm
- Cat. No.: 81001
- Mobile Phase: MeCN:10 mM ammonium formate (pH 3.0) = 5:95
- Flow Rate: 1.0 mL/min
- Detection: Ambient
- Temperature: UV 254 nm
- Sample:
- Acyclovir
- Ganciclovir
- Because HILIC uses a solvent system that is the reverse of what is comment in RP, the peak elution order is reversed on Inspire HILIC
- Polar / hydrophilic compounds that are particularly difficult to retain on RP columns will enjoy maximum retention on HILIC
β-Blockers
- Column: Listed on chromatograms
- Dimension: 150 × 4.6 mm, 5 µm
- Mobile Phase: MeCN:10 mM ammonium formate (pH=3.0) = 85:15
- Flow Rate: 1.0 mL/min
- Detection: UV 220 nm
- Temperature: Ambient
- Sample:
- Propranolo
- Pindolol
- Metoprolol
- Acebutolol
- Nadolol