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MaestroSafe - Model MR-031201 -Nucleic Acid Loading Dye
Size: 1 ml. MaestroSafe Nucleic Acid Loading Dye represent a new and safe class of nucleic acid dye for visualization of double-stranded DNA, singlestranded DNA, and RNA in agarose gels. The dye is developed to replace toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. MaestroSafe Nucleic Acid Loading Dye is noncarcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.
With MaestroSafe Nucleic Acid Loading Dye, you do not need to add any dye to both gel matrix and running buffers. The dye is provided in a form of 6X sample loading dye and it is to be added to your sample only. The dyes completely eliminates any possible contamination of glassware or gel running tank as associated with EtBr. After the electrophoresis, view and document your results as you would do With Etbr Staining Protocols.
MaestroSafe Nucleic Acid Loading Dye emits green fluorescence for dsDNA or ssDNA, and red fluorescence for RNA. This dye has one excitation (470 nm) and two emission spectra (520 nm and 635 nm).
According to the cloning efficiency test, DNA stained with EtBr and exposed by UV light will reduce the efficiency of cloning. The combination of MaestroSafe and LED light shows a better cloning efficiency.
- Cat. No.: MR-031201
- Size: 1ml
- Description: Substitute for loading dye
- Storage: 12 months at 4°C
- Protocol: For laboratory only. Not for clinical applications.
- Prepare a 100ml agarose solution.
- Mix gentle without having any air bubbles.
- Let the solution cool down to 60-70? and cast the gel.
- Mix samples and DNA markers with a MaestroSafe Nucleic Acid Loading Dye at 1:10 dilution rate.
- View the results under traditional UV light after electrophoresis.
- Or use MaestroGen UltraSlim or UltraBright LED Transilluminator for not only good erformance but: health concerns.