PeakTrace:Box - Onsite Basecalling System

The PeakTrace:Box system offers the following features

• Up to 50% more high quality bases per trace.
• Improved appearance of traces.
• Rescue of low quality and failed reads.
• Increased throughput – up to 300% more samples per day.
• Compatible with traces obtained from all ABI DNA sequencers.
• An all-in-one system with an easy to use graphical user interface (Auto PeakTrace) that makes it simple to integrate PeakTrace Basecalling into existing sequencing workflows and LIMS systems.

Simple to use

The PeakTrace:Box DNA sequencing system is both simple to set up and use. The system uses a secure USB Key to provide offline licensing. The USB key can be used with any computer supporting SSE3 (Intel Core processor or better).

The PeakTrace:Box system comes with a Windows graphics software (Auto PeakTrace™) that allows DNA sequencing facility managers to automate the PeakTrace basecalling of ABI files located anywhere on the network. In addition, the Auto PeakTrace software provides a series of tab delimited processing reports (log files) for both the final end users and facility managers. The reports provide users with information on the basecalling improvements as well as why the trace was not able to be improved.

The PeakTrace:Box system is also available as a Linux X86_64 command line version that enables PeakTrace Basecalling to be easily integrated in Linux based LIMS workflows.

Increases trace read length and reduces errors

PeakTrace provides significantly longer and higher quality reads. These improved read lengths mean that fewer sequencing reactions need be performed to complete a sequencing project, thus saving the investigator time and money. In addition, as the PeakTrace Basecaller is compatible with older DNA sequencers like the ABI 377 and 3700, it is able to extend the effective life of these instruments by offering trace read lengths comparable with that obtained from modern instruments such as the ABI 3500 or 3730.

The improved read lengths provided by PeakTrace:Box increase sequence throughput and significantly lower the overall per base cost. These longer read lengths enable sequencing projects (e.g. whole-genome, cDNA clones, etc) to be completed at lower sequence coverage levels, thus offering further cost and time savings.