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PeakTrace - Sanger DNA Sequencing Basecaller and Trace Processor
PeakTrace is a Sanger DNA sequencing basecaller and trace processor that provides better basecalling and improves the appearance and read length of DNA sequencing traces by up to 50%. The PeakTrace Basecaller works with all ABI sequencers including the ABI 377, ABI 310, ABI 3700, ABI 3730, ABI 3730xl, ABI 3100, ABI 3500, and ABI 3130.
The improvement in sequence quality provided by PeakTrace is real, as shown in Figures 2 and 3. These figures show the alignment of the DNA sequence data from the Figure 1 trace file BLAST aligned to the true sequence. The alignable sequence is not only much longer after using PeakTrace (1540 bases aligned) than KB (1379 bases aligned), but the first error is at nucleotide 1084 with the KB Basecaller and at nucleotide 1251 with the PeakTrace Basecaller.