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  5. Pippin Prep - DNA Size Selection System ...

Pippin Prep - DNA Size Selection System for NGS

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The Pippin Prep DNA Size Selection System is a versatile tool designed to optimize library construction for next-generation sequencing platforms, notably endorsed by Illumina and Ion Torrent for specific workflows. This system simplifies the selection of DNA fragments ranging from 100 bp to 1.5 kb, utilizing pre-cast agarose gel cassettes available in various concentrations. This feature ensures accurate targeting for short read sequencing requirements. Capable of processing up to five samples per gel cassette, the system operates efficiently with run times as brief as 30 minutes, ensuring no cross-contamination. The instrument includes built-in optical detection and a CPU, eliminating the need for an external computer. With constant electrophoresis voltage options of 100V or 150V, it supports a stable and reproducible electrophoresis process. Essential standards and reagents accompany the disposable gel cassettes, offering a comprehensive package for effective workflow implementation in DNA size selection.

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Short Read NGS Library Construction – For Inserts from 100bp to 1.5kb

The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross contamination. 

Benefits of the Pippin Prep System:

  • Saves time and effort compared to manual gel purification
  • Sample-to-sample reproducibility streamlines research
  • Higher sample quality improves downstream analysis

Key Downstream Technologies:

  • Paired-end sequencing
  • emPCR
  • RNA-seq
  • ChIP-seq

Pre-Cast Agarose Gel Cassettes for DNA Size Selection

Gel cassettes for DNA selections are available in four agarose concentrations to provide a full range of size selection options between 100bp and 1.5kb.  For smaller fragment sizes, internal standards may be used for higher accuracy and increased sample capacity (from 4 to 5 samples per cassette).  Run times are as little as 30 minutes. View the Pippin Prep cassette reference chart to explore the DNA size selection options and run times.

Gel cassette types and target ranges

  • 3% agarose, 100-250bp
  • 2% agarose, 100-600bp
  • 1.5% agarose, 250bp – 1.5kb

For these downstream technologies

  • miRNA isolation
  • DNA sequencing, RNA-seq, genotyping
  • DNA sequencing, ChIP-seq
  • Mate-pair sequencing

Instrument

The Pippin Prep instrument includes a monitor, keyboard, and mouse. A CPU is built in; an external PC is not required.

  • Pippin Prep Specs 
    Electrophoresis voltage: 100V or 150V constant
    Optical detection: 535 nm excitation | 640 nm emission
    Power requirements: 100-240 VAC, 2.5 A, 50-60 Hz
    Weight: 15 lbs / 7 kg
    Dimensions: 7h x 11w x 21d (in) | 18h x 28w x 53 d (cm)

Cassette Kits
Kits include 10 ea. disposable pre-cast gel cassettes.  All necessary standards and reagents are included with the purchase.