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BioLegend, Inc.
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  5. Purified anti-Streptavidin Antibody

Purified anti-Streptavidin Antibody

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Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including flow cytometry, molecular biology, and bionanotechnology.

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Isotype Control:Purified Mouse IgG2b, κ Isotype Ctrl
Antibody Type:Monoclonal
Host Species:Mouse
Immunogen:Streptavidin
Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation:The antibody was purified by affinity chromatography.
Concentration:0.5 mg/ml
Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C.
Application:

FC - Quality tested

Recommended Usage:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:The 3A20.2 purified antibody is useful as a third step reagent to amplify dim or low signals in many applications. This antibody does not cross-react with avidin.
Product Citations:
  1. Telford W, et al. 2017. Cytometry A. 91:314. PubMed
  2. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  3. Ribechini E, et al. 2017. Blood Adv. 0.699305556. PubMed
  4. Bartsch YC, et al. 2018. Front Immunol. 1.196527778. PubMed
  5. Jiang L, et al. 2018. J Exp Med. 215:1337. PubMed
  6. Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
  7. Aparicio-Domingo P, et al. 2015. J Exp Med. 101084/jm20150318. PubMed
RRID
AB_2564569 (BioLegend Cat. No. 410501)


Structure:52.8 kD tetrameric protein.
Function:Streptavidin binds to biotin with high affinity.
Ligand/Receptor:Biotin
Antigen References
1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343.
2. Zhao X, et al. 2013. J. Anal Methods Chem. 58:1093.
3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135.
4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113.
5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.
Gene ID: NA
UniProt: View information about Streptavidin on UniProt.org