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Purified Streptavidin

Purified Streptavidin

FromBioLegend, Inc.

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Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10^-14 mol/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.

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Formulation:Each vial contains Streptavidin lyophilized in sodium chloride buffer.
Preparation:Reconstitute the content of the vial in distilled water to the desired concentration.
Storage & Handling:For long-term storage, store the lyophilized streptavidin at -20°C or -70°C. After reconstitution, store the solution between 2°C and 8°C for up to 2 weeks, or store in aliquots at -20°C for up to 6 months.
Application:
FC - Quality tested ELISA Capture, ELISA Detection, ICC, IHC-F, IHC-P, IP, WB - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Structure: 52.8 kD tetrameric protein
Distribution: Streptavidin binds to biotin with high affinity
Ligand/Receptor: Biotin
Antigen References

Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343.
Zhao X, et al. 2013. J. Anal. Methods Chem. 2013:581093.
Kaplan DL, et al. 1999. Biomol. Eng. 16:135.
Wilbur DS, et al. 1999. Biomol. Eng. 16:113.
Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.

Gene ID: NA
UniProt: View information about Streptavidin on UniProt.org