Azure - Model Cielo 6 -Real-Time PCR System

Flexible
Engineered for a wide variety of qPCR applications with high sensitivity and wide dynamic range.

Intelligent Workflow
Our user interface allows simple touchscreen assay setup. Saved protocols make assay set up easy and reproducible.

Reliable and Robust
Optics and thermal block are designed to deliver the same uniformity and reproducibility over at least 1000 qPCR experiments.

Connected
Easily operates without use of external. Allows for transfer data via Ethernet, Wi-Fi, or USB.

SAMPLE CAPACITY (WELLS)
96

COMPATIBLE PLATES/TUBES
96-WELL PLATES & TUBES AND 8 OR 12-WELL STRIPS & SINGLE TUBES

REACTION VOLUME
1-150 uL (10-65 uL recommended)

DYNAMIC RANGE
10 LOGS

EXCITATION SOURCE
LED, EXCITATION RANGE: 450–730NM

DETECTION CHANNELS
6

MULTIPLEXING
UP TO 6 TARGETS

REACTION TEMPERATURE
4°C to 99.9°C

MAX. BLOCK RAMP RATE
6°C/sec

AVG. SAMPLE RAMP RATE
4°C/SEC

TEMPERATURE UNIFORMITY
±0.2°C

TEMPERATURE ACCURACY
±0.1°C

CUSTOM DYE/CHEMISTRY
Yes

ONBOARD MEMORY
32GB EQUIVALENT TO 20,000 EXPERIMENTS

CONNECTIVITY
USB, WI-FI, ETHERNET

FOOTPRINT (W/D/H)
12″ X 20″ X 17″ (30.5 X 50.8 X 43.2 CM)

Quickly Set up and Confirm Plate Layout
Each well is color coded by sample type, biological replicate, and technical replicate. Well type and standard curve values are also easily visible.

View Each Individual Amplification Trace by Target or Sample
NIR lasers keep signal high and background low. Our high-performance multiplex NIR lasers and filters deliver robust excitation energy which maximizes emission strength for maximum sensitivity.

2D "Heat Map" of Samples
This feature makes it easy to review your samples and quickly understand your data.

Optical Design Eliminates the Need for ROX
The Cielo’s efficient well-based scanning technology scans multiple data points within each well, eliminating the need for normalization dyes such as ROX.

Amplification curves generated using human genomic DNA template along with human GAPDH primers and a no ROX SYBR Green qPCR mix.

Approximately 100,000 Data Points/Wells Using Total Well Detection Technology
Competitive systems only acquire 1 pixel/well compared to the Cielo’s ability to capture approximately 100,000 pixels/wells, enabling accurate, reproducible and sensitive representation of fluorescent intensities from each qPCR well.

Built for Reproducibility
High-quality engineering includes Peltier-based thermal block system designed for superior thermal cycling and unmatched optical design to ensure every run is accurate and reproducible.

Unparalleled uniformity. Amplification curves for 96 replicates shown on a linear plot

Six-target Multiplex
Comfortably use up to 6 fluorescent channels, which allow for flexible dye usage and broad application. Because Rox is not required, it can also be used as a probe.

• Gene Expression
• Genotyping
• Library Quantification
• Pathogen Detection
• Multiplexing