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RGB Structural Imaging
A central feature of any phenomics screening system is the ability to track growth patterns of plants as they progress through developmental stages and/or through the imposition, onset and recovery from stresses. All PlantScreenTM systems incorporate imaging stations for morphometric analysis of plants, as well as colour analysis. Morphometric and colour analysis may be conducted with equal effectiveness on monocotyledenous and dicotyledonous plants. Scanning is rapid for high throughput analysis, and automated protocols in software correct for any image distortions and provide accurate data of key morphomteric parameters chosen by the operator. The data are collated with data from other imaging stations, if employed, to correlate changes in growth patterns with physiological responses to imposed environmental conditions.
- 2D or 3D scanning mode
- Top view up to 150 cm plant height
- Side view up to 150 cm plant height
- Side view in range 0–360°
- Line scanning mode for side view
- Species oriented analysis (mono and di-cotyledonous plants)
- Static and dynamic analysis
- Homogeneous LED light source
RGB images are scanned with a resolution of 5 to 25MP from three directions (from the sides and from the top). Samples are illuminated with consistent quantity and quality of light. Options are available for the inclusion of novel 3D scanning technology for the most in-depth morphometric reconstruction of plants.
Step 1 (A): Barrel distortion correction
Step 2 (B): Tray detection and cropping
Step 3 (C): Background subtraction
Results (D): Binary and RGB images as input for analysis
- Leaf Area: Number of pixels covered by the plant surface converted to relevant units. Useful for monitoring growth rate.
- Solidity/Compactness: Ratio between the area covered by the plant’s convex hull and the area covered by the actual plant.
- Leaf Perimeter: Particularly useful for the basic leaf shape and width evaluation (combined with leaf area).
- Eccentricity: Plant shape estimation, scalar number, eccentricity of the ellipse with same second moments as the plant (0…circle, 1…line segment).
- Roundness: Based on evaluating the ratio between leaf area and perimeter. Gives information about leaf roundness.
- Medium Leaf Width Index: Leaf area proportional to the plant skeleton (i.e. reduction of the leaf to line segment).
- Circle Diameter: Diameter of a circle with the same area as the plant.
- Convex Hull Area: Useful for compactness evaluation
- Centroid: Center of the plant mass position (particularly useful for the eccentricity evaluation)
- Number of leaves
- Leaf movement – Biomass assessment
- Leaf movement – Relative growth rate