Montana Molecular - Targeted cADDis Live Cell cAMP Assays for Sub-cellular Domains

Montana Molecular offers a suite of targeted cADDis cAMP assays designed to measure cyclic AMP levels within specific sub-cellular domains of living cells. These assays make use of biosensors genetically engineered to localize within distinct cellular compartments like cilia, nucleus, and plasma membrane, providing high precision and specificity. Variants of the assays are available in both green and red fluorescent versions, allowing for robust visualization and quantification of intracellular cAMP dynamics in response to GPCR activation. The use of BacMam vectors ensures consistent delivery and expression of these genetically encoded sensors across a variety of cell lines, facilitating research in cellular signaling. Specific targeting to AKAPs, membrane sites, and cilia enables researchers to discern the spatiotemporal aspects of cAMP signaling pathways, potentially leading to deeper insights into cellular processes affected by such molecular mechanisms.