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Phigenics - Validation Test Products for Validation of Legionella Hazard Control
Phigenics provides comprehensive Legionella testing for all water systems found in commercial, institutional, and industrial facilities. The Phigenics Validation Test (PVT) delivers the fastest & most accurate Legionella testing service by applying the Phigenics patented TimeZero test method for quantitative measurement of viable Legionella and total heterotrophic aerobic bacteria. Results are useful for validation of biological hazard control in potable and utility water systems.
Compared to other methods, the PVT using the TimeZero method is:
- Faster because culturing occurs at “time zero” (t = 0) and results are typically communicated 2-3 days after receipt in the lab
- More accurate because variations due to changes in water samples during shipment are entirely eliminated
- More Comprehensive because:
- Legionella bacteria and total heterotrophic aerobic bacteria (THAB) are obtained from the same sample with one protocol
- PVT test products include both the ISO and PCR methods
- Results are reported electronically with digital images and chain of custody records and are automatically archived in the Phigenics Analytics Platform, providing secure web-based access to results
This unique test is a "time zero" (t=0) dipslide format which means that the sample is inoculated immediately in the field and thereby eliminates inaccuracies due to bacterial growth or decline in water samples during transport to the laboratory. Other conventional tests, including the ISO 11731 Legionella test, are inoculated via spread plating after the drawn water sample is shipped to a laboratory. The delay due to shipping often causes inaccurate results due to sample holding time effects. In fact, scientific survey results show that a statistically-significant number of ISO 11731 spread plate analyses return a false-positive result.
Legionella and Total Bacteria Results
The PVT, using the TimeZero method, has been proven reliable for determining viable cell concentrations of:
- Legionella pneumophila (serogroup 1 and 2-14)
- Non-pneumophila Legionella species
- Total heterotrophic aerobic bacteria (THAB)
- PVT, using the TimeZero test method, provides Legionella species and THAB where the inoculation occurs at “time zero” (t=0) and results are delivered in 2-3 days.
- PVT with ISO, includes the TimeZero test method for Legionella species and THAB plus the Legionella ISO 11731 spread plate method.
- PVT Premium, includes TimeZero test method for Legionella species and THAB, the Legionella 11731 ISO spread plate method, and PCR (a molecular marker negative screen).
- Legionella ISO 11731 (spread plate method) is available as a stand-alone test.
- Legionella Swab Analysis is available for tests of surfaces, such as plumbing fixtures.

Phigenics Analytical Services Laboratories are certified as proficient in the Environmental Legionella Isolation Techniques Evaluation (ELITE) program administered by the Centers for Disease Control and Prevention (CDC) for using the Phigenics patented PVT Test using the TimeZero test method and the ISO 11731 spread plate method.
Phigenics has passed every proficiency test since the beginning of the CDC ELITE program with recurring perfect scores.
Phigenics is an EPA-NELAP certified laboratory for the analysis of drinking water.
