Zeocin™ - Zeocin Solution
Zeocin™ is a formulation of phleomycin D1, a copper-chelated glycopeptide antibiotic produced by Streptomyces CL990. Zeocin™ causes cell death by intercalating into DNA and cleaving it.The action of Zeocin™ is effective on most aerobic cells. Therefore, Zeocin™ is a popular and effective antibiotic for the selection of vectors bearing the Sh ble gene in a variety of cells types (bacteria, eukaryotic microorganisms, plant and animal cells).Resistance to Zeocin™ is conferred by the Sh ble gene product from Streptoalloteichus hindustanus, which inactivates Zeocin™ upon binding to the antibiotic.Sh ble is a small 370bp-sized gene and is carried in a number of InvivoGen’s vectors.Typically, mammalian cells are sensitive to Zeocin™ concentrations of 50-400 µg/ml, and bacteria to 25 µg/ml.
Product concentration:100 mg/ml in solution
CAS number: 11006-33-0
Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Endotoxin level: < 1 EU/mg
Physicochemical characterization: HPLC, pH, appearance
Cell-culture tested: potency validated in Zeocin™-sensitive and Zeocin™-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in Zeocin™-resistant cells
Zeocin™ is supplied as a sterile filtered blue solution at 100 mg/ml in HEPES buffer.
This product is available in three pack sizes:
- ant-zn-1: 10 x 1 ml (1 g)
- ant-zn-5: 50 x 1 ml (5 g)
- ant-zn-5b: 1 x 50 ml (5 g)
Zeocin™ is also supplied as a blue powder:
- ant-zn-1p: 1 x 1 g
- ant-zn-5p: 1 x 5 g
Zeocin™ is shipped at room temperature.
Upon receipt it should be stored at 4°C or -20°C.