MtoZ Biolabs - Model protein-digestion -Protein Hydrolysis Service

Proteins found in nature vary in size from 5 kDa to 400 kDa. The protein hydrolysis process cuts proteins into shorter segments, namely peptides. Protein hydrolysis is a crucial step before peptide mass spectrometry identification analysis, providing a foundation for successful protein identification and characterization, as well as biomarker discovery. Although mass spectrometry (MS) can study intact proteins, smaller peptides are easier to identify proteins and improve protein coverage because protein coverage may decrease due to solubility and heterogeneity. Therefore, the most common proteomics methods often utilize site-specific cleavage sites to produce smaller peptide fragments. Smaller peptides are easier to separate and characterize using high performance liquid chromatography (HPLC) and HPLC-MS.

MtoZ Biolabs offers two methods for protein hydrolysis: (1) in-gel digestion (protein hydrolysis in polyacrylamide gel matrix); (2) in-solution digestion (protein precipitation in chloroform/methanol, then redissolved in urea). Proteins separated by 1D or 2D gel electrophoresis can be hydrolyzed directly in the gel. In-gel digestion is effective and reproducible but more labor-intensive and time-consuming. Compared to in-gel digestion, the in-solution digestion method requires less effort and less time, but proteins hydrolyzed in solution may have low resolubility, possibly leading to some sample loss.

MtoZ Biolabs can handle various sample types, including but not limited to:

1. Proteins from Natural Sources

2. Recombinant Fusion Proteins and Tagged Proteins

3. Different Subtypes of Antibodies

4. Membrane Proteins and Others

1. Flexibility

We offer both in-gel and in-solution digestion strategies.

2. High Coverage and High Accuracy

Our hydrolysis service can greatly improve the accuracy and coverage of mass spectrometry analysis.

3. Wide Applications

Facilitates protein identification and characterization, discovers disease biomarkers, and helps understand biological processes.

4. Comprehensive Proteomics Services

We offer comprehensive proteomics services covering the entire process of proteomics analysis, providing one-stop solutions for your omics needs.

MtoZ Biolabs is an integrate contract research organization (CRO) providing advanced proteomics, metabolomics, bioinformatics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. The name of MtoZ represents “mass to charge ratio” in mass spectrometry analysis, as most of our services are provided based on our well-established mass spectrometry platforms. Our services allow for the rapid and efficient development of research projects, including protein analysis, proteomics, and metabolomics programs.

MtoZ Biolabs is specialized in quantitative multiplexed proteomics and metabolomics applications through the establishment of state-of-the-art mass spectrometry platforms, coupled with high-performance liquid chromatography technology. We are committed to developing efficient, and effective tools for addressing core bioinformatics problems. With a continuing focus on quality, MtoZ Biolabs is well equipped to help you with your needs in proteomics, metabolomics, bioinformatics, and biopharmaceutical research. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample.

Email: marketing@mtoz-biolabs.com