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Shotgun Metagenomic Sequencing Services
Shotgun metagenomic sequencing provides a detailed picture of the composition of microorganisms in a sample. As opposed to amplicon-based methods like 16S rRNA sequencing, shotgun sequencing covers microorganisms from different kingdoms of life and increases the resolution by providing information on strain level variation. In addition to providing detailed information on which organisms are found and their relative abundance, this sequencing solution also profiles the genetic content of the microorganisms thereby providing information on the functional potential of the microbiome in a sample. Simply put, it tells you both who is there and what they can do. By applying our data processing and biostatistical platform, we can evaluate if a certain test condition, such as a new food ingredient, affects the composition and function of the microbiome, and detect which specific genes and pathways are involved.
- Higher resolution as compared to amplicon sequencing (16S and ITS). Shotgun sequencing can provide information on organisms down to strain level while 16S/ITS is limited to genera or, in ideal cases, species-level information.
- Information on both taxonomy and function (genes and pathways).
- DNA extraction
- Shotgun metagenomic sequencing on a HiSeq Illumina platform
- Data quality evaluation and quality filtering to prepare the data for microbiome profiling
- Microbiome profiling – calculation of the relative abundance of specific organisms and functional features in the samples
- Statistical analysis
For each project, a private project folder is made available on the Biomcare server, where all resulting data, statistical results, illustrations, reports, etc. are available for download.
Sample collection barcodes, tubes, or kits
Depending on the type of tissue you collect, we will ship barcode stickers or barcode-labeled tubes. For larger clinical projects, we can provide collection kits if requested.
Data
- Raw sequencing data in fastq format
- Quality filtered data in fastq format
Results reports
Three reports are generated with all necessary information on sequencing, data processing, and statistical analysis, and all resulting results, illustrations, and tables.
- A report describing the performed sequencing, data evaluation, and processing
- A report describing the generated microbiome profiles, and first microbiome evaluations
- A report describing the statistical analysis performed and the results, including illustrations and tables.