Version D1000 -ScreenTape

The Agilent 2200 TapeStation system provides automated, fast and reliable DNA, RNA and protein electrophoresis for up to 96 samples using pre-packaged reagents and minimal manual handling. The Agilent D1000 and High Sensitivity D1000 ScreenTape assays have been developed for the separation and analysis of DNA fragments from 35 bp to 1000 bp for the 2200 TapeStation system. This Technical Overview focuses on the performance of both D1000 assays with respect to the accuracy and precision of quantifi cation and sizing, as well as the sensitivity of these assays. Data analysis for quantifi cation and molarity determination was further directly compared to the respective assay for the Agilent 2100 Bioanalyzer system. Table 1 summarizes the analytical specifi cations of both the D1000 and High Sensitivity D1000 ScreenTape assays.