Determination of proteins concentrations and their molecular weights - Environmental
INTRODUCTION
The method enables fast separation of proteins according to their molecular weights (Mw) with subsequent protein quantification in pr otein-containing samples.
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Capillary electrophoresis for the separation and dete rmination of proteins is based on the differential migration of SDS-protein complexes in a narrow fused-s ilica capillary filled with a low viscous gel, under the influence of the applied electric field. Detection of proteins is performed based on their own absorbance at 220 nm. Due to the presence of a low viscous gel SDS-protein complexes are separated only according to their Mw and thus it enables also to determine Mw of an unknown protein(s).
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