Separation Methods Technologies, Inc. (SMT) products

Particle Size: 1.5 µm, Pore Size: 100Å, Length: 100 mm, I.D.: 2.1 mm. SMT offers different C18 phases of varying carbon loads for optimal selectivity: OD Series: SMT SAM-C18 phase with the highest functional ligand coverage confirmed with carbon analysis results of 24% carbon load. In these series, a very high density of the functional ligand, octadecyl (C18) molecule, is achieved through meticulous mixture of the C18 with proprietary spacer molecules to ensure maximum coverage. These phases are very hydrophobic and are designed to tolerate usage in very aggressive pH conditions and high temperatures.

Particle Size: 5 µm, Pore Size: 100Å, Length: 100 mm, I.D.: 4.6 mm. SMT-C30 columns consist of Triacontyl as the functional ligand. The columns offer selectivities that are much different from C18 reversed-phase columns when applied to separation of carotenoid and related compounds. Carotenoids consist of very diverse groups of molecules that include nonpolar hydrocarbons and polar xanthophylls. These compounds have geometric and positional isomers with very subtle molecular differences that can pose challenges in separation. Previous efforts to separate these compounds with available C18 and other reversed phases have been unsatisfactory. SMT-C30 ligand provides sufficient interactive sites for complete partitioning of these positional isomers.

Particle Size: 10 µm, Pore Size: 100Å, Length: 150 mm, I.D.: 4.6 mm. Chiral compounds, or enantiomers have identical molecular structures that are related as mirror images of one another much like a left hand is related to a right hand. Rapid and accurate stereochemical resolution of enantiomeric molecules has become a challenge in various aspects of pharmaceuticals and drug discoveries. A chiral column may contain one form of an enantiomeric compound immobilized on the surface of a packing material.

Particle Size: 5 µm, Pore Size: 100Å, Length: 150 mm, I.D.: 4.6 mm. SMT CIB-IgG are silica-based packing materials designed for for both high performance and low-pressure purification of protein A. In production of the IgG affinity-type columns, SMT utilizes its proprietary technique of self-assembled monolayer (SAM) of bonding to first immobilize unique mixed phases of appropriate silane ligands that are capable of effectively anchoring IgG on silica substrates. This total coverageTM technology ensures complete elimination of non- specific binding sites on the silica surface. The rigidity of silica particles enables the columns to be used under high performance chromatographic conditions as well as for ru- tine low-pressure applications.