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Background:  Lipid droplets are coated with a layer of proteins that regulate their growth and metabolism.  The major lipid droplet-associated protein in adipocytes is perilipin [1].  Mice in which the endogenous gene for perilipin is knocked out have a lean phenotype due to reduced accumulation of fat [2,3], suggesting that perilipin may have a “gate-keeper” function under basal conditions to protect the triglycerides from metabolism by lipases.  Upon activation of lipolytic pathways, perilipin is subject to phosphorylation at serine 497 and 522 by cyclic-AMP-dependent protein kinase, and phosphorylation of perilipin helps to recruit hormone sensitive lipase (HSL) to the lipid droplets. Amount:  100 µL.

Background:  Lipid droplets are coated with a layer of proteins that regulate their growth and metabolism.  The major lipid droplet-associated protein in adipocytes is perilipin [1].  Mice in which the endogenous gene for perilipin is knocked out have a lean phenotype due to reduced accumulation of fat [2,3], suggesting that perilipin may have a “gate-keeper” function under basal conditions to protect the triglycerides from metabolism by lipases.  Upon activation of lipolytic pathways, perilipin is subject to phosphorylation at serine 497 and 522 by cyclic-AMP-dependent protein kinase, and phosphorylation of perilipin helps to recruit hormone sensitive lipase (HSL) to the lipid droplets. Amount:  100 µL.

This cell extract serves as a control for Western blotting procedures (e.g., for use with Anti-p-Perilipin 1-serine 522, Cat. 4856); the extract was derived from cells that were not exposed to lipolysis-activating agents.

KIC provides high throughput in situ “optical cytometry”. Vala’s flagship kinetic image cytometer (IC200-KIC®) with high-throughput automation and analytics is unmatched in visualizing and measuring subtle disease effects in individual cells.