Hands-on HPLC & LC-MS

This course provides the theory of HPLC and LC-MS essential to any participant, along with the hands-on practical element in the laboratory to practice and re-enforce the theoretical knowledge. These courses are 50% theory and 50% practical in a lab environment. After an introduction to liquid chromatography, this course addresses solvents & buffers (the mobile phase), pumps, isocratic and gradient elution, sample introduction, analytical columns, HPLC detectors, mass spectrometry including tuning, ionisation, mass analysers, ion detector and data analysis. This course combines the five individual hands-on days covering HPLC Theory and Methods, HPLC Maintenance, LC-MS Theory and Methods, LC-MS Maintenance and HPLC & LC-MS Troubleshooting. Who is this course for? This course is suitable for all levels of knowledge; however for complete beginners it is advisable to attend the Absolute Basics of HPLC & LC-MS first to get a good basic overview of liquid chromatography and LC-MS.