Chromatography Articles

  • Photodegradation of diclofenac in aqueous solution by simulated sunlight irradiation: kinetics, thermodynamics and pathways

    Diclofenac (DCF) is one of the most frequently detected pharmaceuticals in various water samples. This paper studied the effects of aquatic environmental factors (pH, temperature and dissolved organic matter) on photodegradation of DCF under simulated sunlight. The results demonstrate that degradation pathways proceed via pseudo first-order kinetics in all cases and the photodegradation of DCF ...


    By IWA Publishing

  • The Clog Blog

    Clogging and over pressuring are definitely problematic for Liquid Chromatography columns. Back in 2005 John Dolan’s sage advice to chromatographers to make an LC column last forever was simple, “Never open the box!” As John pointed out, this is not practical and I have to agree. But as an LC column manufacturer I have seen remarks from customers regarding clogging as though it ...


    By Restek Corporation

  • Webinar Recap: “Developing, Testing, and Troubleshooting Chromatography Method Transfer”

    Hi, I’m Tom Wheat, Principal Scientist at Waters, and I recently hosted a webinar entitled, “Developing, Testing and Troubleshooting Chromatography Method Transfer.” Over the course of the webinar, we discussed the transfer of chromatographic methods that occurs at many stages in the development and production of a product. In its simplest form, the method is moved from one ...


    By Waters Corporation

  • High-Resolution Ultrahigh-Pressure Long Column Reversed-Phase Liquid Chromatography for Top-down Proteomics

    Separation of proteoforms for global intact protein analysis (i.e. top-down proteomics) has lagged well behind what is achievable for peptides in traditional bottom-up proteomic approach and is becoming a true bottle neck for top-down proteomics. Herein, we report use of long (≥1 M) columns containing short alkyl (C1-C4) bonded phases to achieve high-resolution RPLC for separation of ...

  • Novel mixed-mode stationary phases for chromatographic separation of complex mixtures of decomposed lignin

    Abstract Electrochemical degradation of lignin results in a large number of unknown products, formed by both oxidative and reductive decomposition pathways, which have previously been studied by direct mass spectrometry experiments without prior liquid chromatography separation, revealing the presence of many isobaric molecules. In this study, novel phosphonium-based ionic liquid stationary ...


    By SusChem

  • Top 16 for 2016 - Restek LC article makes the cut

    If you are an avid chromatographer you likely subscribe to LC/GC magazine or its popular electronic version, E-Separation Solutions. It is definitely the place to go for news and information related to all things Chromatography. Over the years Restek has had many an article published in LC/GC and, full disclosure, we advertise there as well. Who in the business doesn’t? It is the place ...


    By Restek Corporation

  • How a contract research lab advanced its polymer characterization capabilities

    Realizing the Advantages of Ultra-High-Performance Size Exclusion Chromatography Compared to GPC Intertek is a global provider of quality and safety services designed to help businesses improve products, assets and processes, overcome market constraints and reduce risks. From auditing and inspection, to testing, training, and certification, Intertek enables customers to meet end ...


    By Waters Corporation

  • Photocatalytic degradation of tartrazine dye using CuO straw-sheaf-like nanostructures

    Straw-sheaf-like CuO nanostructures were fruitfully synthesized using a chemical precipitation approach for the photocatalytic degradation assessment of tartrazine. Phase identification, composition, and morphological outlook of prepared CuO nanostructures were established by X-ray diffraction and scanning electron microscopy analysis. The photocatalytic performance of the synthesized CuO ...


    By IWA Publishing

  • Bioremediation of synthetic fatliquors under microaerobic condition

    Synthetic fatliquors are useful as a fatliquoring agent, flotation agent and emulsifying agent in a wide range of industrial applications such as leather, pharmacy and farm chemicals. These fatliquors remain recalcitrant to natural biota in existing treatment plants. In the present study, the isolated microaerophilic Serratia sp. HA1 strain CSMB3 is capable of utilizing structurally different ...


    By IWA Publishing

  • Degradation and toxicity depletion of RB19 anthraquinone dye in water by ozone-based technologies

    This research investigated the discoloration and mineralization of Reactive Blue 19 (RB19) anthraquinone dye by single ozonation, single UV radiation and ozonation jointed with UV radiation (O3/UV). The problem was approached from two points of view: with the objective of color removal or the mineralization of solution. In each case, the optimum operating conditions were different. Ozonation ...


    By IWA Publishing

  • Separation of citral isomers from lemongrass oil

    Reveleris® Prep: Purification with flash chromatography followed by a prep-HPLC isolation of citral isomers Lemongrass is cultivated around the world. It's mainly grown for its oils, which have a number of uses including for vitamin A, perfumes, insect spray, cosmetics, perfumes, food and drinks. Lemongrass is also enjoyed as a tea throughout the world. Lemongrass oil contains several ...


    By BÜCHI Labortechnik AG

  • Development of a Cleaning Validation Method for MICRO® A07 Citric Acid Cleaner Using HPLC-CAD

    Abstract A high performance liquid chromatography (HPLC) method with a Corona Charged Aerosol detector for the cleaning validation of MICRO* A07 Citric Acid Cleaner residue has been developed. The pooled average repeatability of 3 replicates at 6 concentration levels expressed as RSD was 1.3 % while the pooled average intermediate precision (intra-day repeatability in 3 days) of 3 replicates ...

  • A brief introduction to DiatoSorb-W

    DiatoSorb-W is a diatomaceous earth material that Restek will sell in bulk and in packed columns, uncoated and coated (with a liquid phase of your choice), which will replace Chromosorb-W packings. For those of you who may not know, the “W” stands for “white”, and is simply the color of the untreated, uncoated material. If your method references USP S1A, or Chromosorb-W/AW ...


    By Restek Corporation

  • Tips to maintain your HPLC & UHPLC systems and columns

    Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your ...


    By Waters Corporation

  • Glyphosate and AMPA in drinking water

    Carcinogenic or not? The broad-spectrum herbicide glyphosate is used all over the world in agriculture. Alongside farming, the chemical is also used for weed-killing in domestic gardens and in public and private spaces kept free from «vegetal invasion», such as railway tracks. Glyphosate has been used since the 1970s in pesticides and was hitherto thought to be harmless at ...


    By Metrohm AG

  • Comparison of simple ozonation and direct hydrogen peroxide processes in TNT removal from aqueous solution

    Oxidation of 2,4,6-trinitrotoluene (TNT) using simple ozonation and direct hydrogen peroxide was studied. An analytical method based on the HPLC (high performance liquid chromatography) system was used for TNT detection. The influence of parameters such as pH of the solution (3–10), initial TNT concentration (10–100 mg/L), dosage of ozone (0.2–1 g/hr), H2O2/TNT molar ratio (250–1,000), ...


    By IWA Publishing

  • Iridium nanoparticles with high catalytic activity in degradation of acid red-26: an oxidative approach

    Nanocatalysis using metal nanoparticles constitutes one of the emerging technologies for destructive oxidation of organics such as dyes. This paper deals with the degradation of acid red-26 (AR-26), an azo dye by hexacyanoferrate (abbreviated as HCF) (III) using iridium nanoparticles. UV-vis spectroscopy has been employed to obtain the details of the oxidative degradation of the selected dye. ...


    By IWA Publishing

  • Microwave synthesis of pure and doped cerium (IV) oxide (CeO2) nanoparticles for methylene blue degradation

    Cerium (IV) oxide (CeO2), samarium (Sm) and gadolinium (Gd) doped CeO2 nanoparticles were prepared using microwave technique. The effect of microwave irradiation time, microwave power and pH of the starting solution on the structure and crystallite size were investigated. The prepared nanoparticles were characterized using X-ray diffraction, FT-Raman spectroscopy, and transmission ...


    By IWA Publishing

  • What is the pressure limit for my LC column?

    This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ...


    By Restek Corporation

  • Is Microflow LC a Reality or Perception?

    Microflow or nanoflow chromatography has been used mostly in the academic and fundamental research environment in the past for its various advantages over analytical liquid chromatography, but rarely in the industrial settings. This is most likely because many of us believe it is a pain-in-the-neck technique to perform! But is this a reality or just a ...


    By Waters Corporation

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