Cytiva
Cytiva - Membrane Filtration (MF) Technique
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The membrane filtration (MF) technique is a versatile and useful method for concentrating organisms from an aqueous sample onto the surface of a filter for reliable detection and accurate enumeration. The filter is then placed on a culture medium for culture and analysis.
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- Larger sample for better detection limits
- Separate organisms from growth inhibitors
- Selection of membrane pore size and rinse buffer
Fast and Simple
- Use membrane to isolate and culture
- Save time
- Avoid risk of contamination during transfer of sample
Improved Sensitivity
- Concentrate large sample volumes
- Better detection limits
Remove Growth Inhibitors
- Use rinse buffers to neutralize inhibitory substances
- Separate inhibitory substance from target organisms
Isolated Colonies
- Discrete colonies grow on membrane surface
- Easily count, identify and select colonies
- Place membrane and secure funnel
- Filter and rinse sample
- Plate membrane and incubate
- Count colonies
- Large sample volumes
- Samples with low counts
- Samples with inhibition
- Versatile: membrane, media, rinse buffer selection
- Large range of samples
MF technique is not suitable with high-particulate samples. For high-particulate samples, choose the Most Probable Number (MPN) method.
Whatman mixed cellulose membranes for MF technique
- Pore sizes: 0.2, 0.45, 0.6, 0.8, 1.2 μm
- Colors: white, green, black
- Grid lines: black, white
- Sterile or non-sterile
- Individual or dispenser pack
- 47 or 50 mm (more sizes non-sterile)