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Microbubble Leukopak Human T Cell Isolation Kit – GMP
With quality standards built into the design and manufacturing process at ever step, use our Microbubble Leukopak Human T Cell Isolation Kit – GMP Grade to isolate truly untouched T cells directly from leukapheresis material without the need for lysis or density gradient centrifugation. The Microbubble Leukopak Human T Cell Isolation Kit – GMP Grade was developed with BACS™ Microbubbles to negatively select untouched T cells from leukpak material. Non-T cells are targeted and removed with antibodies recognizing CD14, CD16, CD19, CD20, CD36, CD56, CD123, CD235ab via negative selection. Isolated T cells are suitable for a wide variety of downstream assays, including cell culture, flow cytometry, scRNA-Seq, or other functional studies. The Microbubble Leukopak Human T Cell Isolation Kit – GMP has the capacity to isolate T cells from an average half-leukopak (12.5 x 109 total cells).
- GMP grade – Our GMP grade allow you to have peace of mind, with stringent documentation and processes, traceability, and well-defined standard.
- Enjoy higher recovery – Industry leading recovery of T cells from leukopak material allows you to get the most out of your leukopaks, limiting donor recalls.
- Shorter processing time – From leukopak bag to purified cells in under an hour enables greater productivity, adding flexibility to your day.
- No dedicated equipment, no magnets, or columns – Only general tools and equipment are needed for our quick and easy workflow.
The Microbubble Leukopak Human T Cell Isolation Kit – GMP streamlines T cell isolations by eliminating the need for excess equipment and time-consuming sample preparation (i.e., Ficoll gradients or lysis) – allowing you more time to spend focusing on what matters, your science. By eliminating the need for pre-processing, the Microbubble Leukopak Human T Cell Isolation Kit – GMP minimizes handling steps and consistently delivers more isolated T cells. Get the most our of your time and material with the Microbubble Leukopak Human T Cell Isolation Kit – GMP.
Mechanism of Action: Leukopak samples contain a milieu of immune cells (i.e., white blood cells), plasma, platelets, and red blood cells. The Direct from Leukopak T Cell Isolation Kit uses a negative selection method to enrich T cells by removing unwanted cell populations. These unwanted cells are labeled with biotin-CD14, CD16, CD19, CD20, CD36, CD56, CD123, CD235ab. BACS™ Streptavidin Microbubbles are mixed into the sample where they bind to the labeled cells and gently float them to the surface. Untouched T cells in their original state are subsequently harvested.
How it Works: The Human T Cell from Leukopak Biotin- Antibody Cocktail is added to the leukapheresis product to label unwanted cell populations. BACS™ Streptavidin Microbubbles are added to the sample and bind to the biotin-labeled cells. Due to their inherent buoyancy, the microbubbles quickly and gently float the non-T cells to the surface. Following centrifugation, the T cells are collected.
Additional Information:
- Storage: 4°C
- Cell Type: T Cells
- Sample Species and Source: Human Leukopaks
- Cell Separation Method: Negative Selection
- Capacity: 12.5 x 10? total cells (enough to process an average half-leukopak)
This kit comes with:
- BACS™ Streptavidin Microbubbles
- Human T Cell from Leukopak Biotin-Antibody Cocktail
- Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA)
Recommended for Best Results
- 20 rpm tube rotator for mixing (e.g., Thermo Scientific cat#: 88881002)
- Centrifuge (swinging bucket rotor strongly preferred)
- Vacuum aspirator
The Microbubble Leukopak Human T Cell Isolation Kit – GMP is designed to enrich T Cells from leukopaks. Akadeum’s novel Buoyancy Activated Cell Sorting (BACS™) Microbubbles enable processing, limiting the unnecessary loss of cells. The T cell isolation protocol is fast and gentle on cells, taking just one hour and not requiring upfront processing or magnets or columns. The result is a population of highly pure T cells that are ready for downstream use.
Elevated Solutions for Sample Preparation
- Ficoll- and lysis-free cell separation for the isolation of truly untouched T cells.
- Fast and easy workflow; leukopak bag to cells in under 60 min.
- Maximum yield of T cells without compromising on high purity.
Elegant in Simplicity, Powerful in Application
BACS™ Microbubble technology harnesses the power of flotation to provide a fast, easy, and gentle T cell isolation without the need for pre-processing of the leukopak material. Our novel technology couples performance, ease, and quality, bringing cell separation to the next level.
Experience the benefits of a microbubble workflow. Akadeum’s microbubbles are:
- Gentle on cells: Harnessing the power of buoyancy, BACSTM Microbubbles swiftly and gently remove unwanted cells via flotation.
- Fast, reliable, and scalable: The simple BACSTM Microbubbles protocol delivers reproducible results that can be easily scaled for greter numbers of samples or larger volumes.
- Equipment-free: Free yourself from worring about the maintaining magnets, losing cells to clogged columns, or sacrificing space in the biosafety hood.
Untouched T cells were isolated from leukopak material using the Leukopak Human T Cell Isolation Kit. Isolated T cells were labeled with CD45-FITC and CD3-APC Fire/750. The fluorescently labeled cells were analyzed by flow cytometry. Dead cells and debris were excluded from the analysis.