Assays Articles

  • Evaluation of an automated luminescent bacteria assay for in situ aquatic toxicity determination

    A new system for monitoring toxicity TOXcontrol® (MicroLAN BV, The Netherlands) has been used to assess the toxicity of a selection of priority or emergent compounds in the laboratory. In this study, inhibition curves and EC50 – Effective Concentration causing 50% inhibition – of selected compounds (including pesticides, pharmaceuticals, surfactants and metals commonly detected in ...

  • Online Total Bacteria Monitor compared with ATP and Flow Cytometry

    Alkaline Phosphatase Enzyme Activity Measurement as a Robust on-line Monitoring Assay for Water Quality Assessment. Micro organisms maintain their growth by producing enzymes in order to provide low molecular weight inorganic compounds. These compounds could be taken up into the cells from high molecular weight organic compounds which cannot be directly utilized by bacteria. Microbial ...

  • Research Spotlight: Pierre Baillargeon, Scripps Research

    This week’s Research Spotlight focuses on Pierre Baillargeon, a senior robotics engineer in the Department of Molecular Medicine at the Scripps Research Institute. His work mostly centers around using 3D printers and automation to improve assay development, compound management, and ultra-high throughput ...


    By GA International

  • Virus detection: Fast, sensitive, and cost-effective with electrochemical testing

    This white paper discusses the possibilities of rapid, point-of-care virus testing using inexpensive and easy-to-use screen-printed electrodes that are produced with ISO 13485 certification ("Manufacturing of sensors for medical devices"). Compared with other methods, such as virus isolation cultures, enzyme-linked immunosorbent assay (ELISA), and other molecular methods, including polymerase ...


    By Metrohm AG

  • Glycerol Detection Enzyme - Case Study

    BACKGROUND When biodiesel is made - from any feedstock - approximately 10% of the product will be glycerol (also called glycerin). Glycerol has many uses, but it destroys engines because it's a sugar alcohol, and therefore must be removed from biodiesel before it can be used or sold as fuel. ASTM International has set a limit of 0.02% glycerol in biofuels (ASTM Standard D6751). ASTM's ...


    By NECi Superior Enzymes

  • Nitrate Biosensor - Case Study

    BACKGROUND In 1998, Dr. Bill Campbell and his team published a peer-reviewed journal article in Analytical Chemistry on the construction and characterization of Nitrate Reductase-Based Amperometric Electrode and Nitrate Assay of Fertilizers and Drinking Water. This publication set the stage for the nitrate biosensor project. In this publication, we found that nitrate reductase was ...


    By NECi Superior Enzymes

  • Indoor Air Quality (IAQ) Surveys in High-Rise Buildings

    We have developed a distinctive approach to evaluating individual tenant spaces and entire high-rise buildings for air quality, including molds and chemicals. Sick Building Syndrome (SBS) and Building Related Disease (BRD) are more likely in large commercial spaces because of the complexity of the HVAC systems, variety of tenants, space constraints and the fact that they are in dense metropolitan ...


    By Atlantic Environmental, Inc.

  • Charles River to Expand Biologics Capabilities in Edinburgh - Case Study

    In order to meet the growing needs of the biologics testing industry, we're excited to announce the expansion of our biologics site in Edinburgh. GMP testing services located in Tranent will be moving to Riccarton. The move will be fully complete by early 2020, and the new facility will have double the capacity to accommodate your testing needs. All current sponsor methods and staff will be ...


    By Charles River

  • Identifying rAAV Residual Impurities with LC-MS - Case Study

    Gene therapies involving recombinant adeno-associated viral (rAAV) vectors have been approved by FDA, EMA, and used in well over 100 clinical trials, demonstrating efficacy against many genetic diseases. Improvements to ...


    By Charles River

  • Hormone Potency Assays Aid Reproductive Health - Case Study

    Gonadotropins regulate the development of ovarian follicles and stimulate the production of sperm, making them essential for reproduction. They are used therapeutically for assisted reproduction in humans and to induce ovulation and superovulation in various mammals. The potency of gonadotropins is required to be evaluated accurately to ensure there is consistency between batches to allow ...


    By Charles River

  • Water Quality and Toxicity Assessment in Koronia Lake - Greece

    Abstract :Lake Koronia, located in Northern Greece, is an environmentally sensitive area protected by Ramsar Convention. However, COD values higher that 900 mg/l and nitrate-nitrogen concentration higher than 10 mg/l were measured in the lake water. Furthermore, toxicity bioassays based onDaphnia magna, resulted in mortality values of test species higher than 40% in the lake water. The poor water ...


    By Microbiotests Inc.

  • Alternative Assays for Routine Toxicity Assessments : a Review

    Abstract : The number of available aquatic toxicity tests is large and increases every year. For certain types of applications such as the routine screening of large numbers of environmental samples, standard conventional tests may be perceived as being unpractical. This has lead to the development of a (increasing) number of alternative small-scale toxicity tests also called microbiotests. The ...


    By Microbiotests Inc.

  • Ecotoxicity Assessment of Landfill Leachates using a Battery of Bioassay and Toxicity Characterization of the Leachates

    Abstract : The western part of Teshima Island in the Seto Inland Sea (Japan) had been used as an illegal dumping ground for more than 10 years and the accumulated waste was estimated to be about 510.000 tons. There has been no systematic toxicity assessment conducted on this waste. In this study, a battery of bioassays was applied to the collected leachates. Toxicity of the leachates was ...


    By Microbiotests Inc.

  • Bioassays used for Detection of Ecotoxicity at Contaminated Areas

    Abstract : Ten bioassays were used for the evaluation of acute, (sub)chronic toxicity and genotoxicity of an unknown soil from a military base and six model soils that were contaminated at former military bases with the major pollutants : kerosene, petroleum and used motor oil. The test species represented algae and higher plants, invertebrates and bacteria. The ability to detect toxicants in ...


    By Microbiotests Inc.

  • Toxicity Assessment Assays in Greece

    Abstract :In this study the results of toxicity assessment assays in the area of Thessaloniki in Greece are presented.Daphnia magnatests were used for the assessment of freshwater and wastewater andArtemia salinatests for the assessment of seawater. There was increased toxicity in the seawater of inner Thermaikos gulf as well as in the freshwater of Koronia lake. Measures, taken to prevent toxic ...


    By Microbiotests Inc.

  • Modelling of Human Acute Toxicity from Physicochemical Properties and Non-Vertebrate Acute Toxicity of the 38 Organic Chemicals of the MEIC Priority List by PLS Regression and Neural Network

    Abstract :Linear and non-linear modelling of human acute toxicity (as human lethal concentrations; HLCs) of the 38 organic chemicals from the 50 priority compounds of the Multicentre Evaluation ofIn VitroCytotoxicity (MEIC) programme was investigated. The models obtained were derived either from a set of 23 physicochemicals properties of the compounds of from their acute toxicities to five ...


    By Microbiotests Inc.

  • Comparison of the Conventional Algal Assay and the Algaltoxkit FTM Microbiotest for Toxicity Evaluation of Sediment Pore Waters

    Abstract : Conventional algal tests have not been utilized to their full potential for routine toxicity assessments. This is mainly due to the complexicity of these assays and the fact that many operators are still encountering practical difficulties. In view of the need for a cost-effective alternative to standard algal assays for routine testing, a new culture/maintanance free algal test was ...


    By Microbiotests Inc.

  • Microbiotests for Cyanobacterial Toxins Screening

    Abstract : The development of massive blooms of cyanobacteria (blue-green algae) in eutrophicated fresh and salt waters is an actual problem in many countries. Moreover, many strains of cyanobacteria produce toxins with a specific mode of action, the most studied being hepatotoxins (such as e.g. microcystin) and neurotoxins (e.g. anatoxin-a). Toxicity testing of crude extracts of cyanobacterial ...


    By Microbiotests Inc.

  • Cyst-Based Toxicity Tests. - VII. Evaluation of the 1-h Enzymatic Inhibition Test (Fluotox) with Artemia nauplii

    Abstract : The newly developed 1 h enzymatic inhibition bioassay (Fluotox) was applied to toxicity testing with the larvae of the brine shrimp Artemia. The method consists of visual observation of in vivo inhibition of an enzymatic process using a fluorigenic enzyme substrate. The 1 h Fluotox test and the conventional 24 h and 48 h LC50 toxicity tests were conducted in parallel on 8 different ...


    By Microbiotests Inc.

  • Cyst-Based Toxicity Tests. - VI. : Toxkits and Fluotox Tests as Cost-Effective Tools for Routine Toxicity Screening

    Abstract : During the last two decades microbiotests have been developed which are independent of recruitement, maintenance and/or culturing of live stocks of test organisms. Culture and maintenance free microbiotests have been worked out in the Laboratory for Biological Research in Aquatic Pollution at the University of Ghent, with selected aquatic invertebrates. The new approach is based on the ...


    By Microbiotests Inc.

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