UHPLC Articles

  • What is the pressure limit for my LC column?

    This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ...


    By Restek Corporation

  • Out with the old, in with the new…

    We’re all familiar with the adage, “Out with the old, in with the new,” but we probably tend to think of it as it applies to clothes for donation or past relationships, and maybe not in terms of updating our old laboratory equipment. After attending my first WCBP conference, held in Washington, D.C. during January, and hearing life cycle management and ICH Q12 being discussed ...


    By Waters Corporation

  • Quantitative LC-MS/MS Analysis of Microcystin Cyclopeptides: EPA Method 544

    Accurate and sensitive characterization and quantification of a structurally diverse class of microcystin cyclopeptides was accomplished using UHPLC-MS-MS. In this analysis, microcystins-RR, YR, and LR were measured using a Nexera UHPLC with a LCMS-8050 triple quadrupole MS detector with ESI ionization in accordance with EPA Method 544. The results of this study ...

  • High Throughput LC-MS-MS Analysis of Carbendazim in Orange Juice

    Highly sensitive analytical methods are necessary to prevent contaminated produce from entering the food supply. In order to help move fresh imports through customs without delay, methods must be rapid and cost effective, in order to test imports thoroughly. LC-MS offers the highly sensitive, robust measurement needed, while ultra high performance liquid chromatography enables high speed ...

  • Increased throughput liquid chromatography and reduced solvent consumption for the determination of isoflavones by UHPLC

    All plant foods are complex mixtures of chemicals including both nutrients and biologically active non-nutrients, referred to as phytochemicals. Soy is known for having high concentrations of several physiologically-active phytochemicals, including isoflavones, phytate (inositol hexaphosphate), saponins, phytosterols and protease inhibitors. The isoflavones are what makes soy unique. Soy ...


    By PerkinElmer, Inc.

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