HPLC Articles

  • What can I use to filter my mobile phase?

    If you are using mobile phase that contains salts or, generally, any components that are weighed out for its preparation, you will need to filter the solution before using with HPLC or UHPLC. The most common type of filtration is membrane microfiltration. We sell an extensive group of products for this and they are discussed very well in a blog ...


    By Restek Corporation

  • Separation of citral isomers from lemongrass oil

    Reveleris® Prep: Purification with flash chromatography followed by a prep-HPLC isolation of citral isomers Lemongrass is cultivated around the world. It's mainly grown for its oils, which have a number of uses including for vitamin A, perfumes, insect spray, cosmetics, perfumes, food and drinks. Lemongrass is also enjoyed as a tea throughout the world. Lemongrass oil contains several ...


    By BÜCHI Labortechnik AG

  • Why Biphenyl should be your first choice in LC.. A perspective from a “ GC” specialist..

    Being in GC for 38 years it made me wonder why in LC mostly C18 columns are used for developing a method. I never understood this as its against the basic rules for selecting the optimal stationary phase. In LC we see that about 95% of the applications is done using reversed phase materials, especially the C18 type. There are many C18 types available, and they all show a different separation ...


    By Restek Corporation

  • Development of a Cleaning Validation Method for MICRO® A07 Citric Acid Cleaner Using HPLC-CAD

    Abstract A high performance liquid chromatography (HPLC) method with a Corona Charged Aerosol detector for the cleaning validation of MICRO* A07 Citric Acid Cleaner residue has been developed. The pooled average repeatability of 3 replicates at 6 concentration levels expressed as RSD was 1.3 % while the pooled average intermediate precision (intra-day repeatability in 3 days) of 3 replicates ...

  • A brief introduction to DiatoSorb-W

    DiatoSorb-W is a diatomaceous earth material that Restek will sell in bulk and in packed columns, uncoated and coated (with a liquid phase of your choice), which will replace Chromosorb-W packings. For those of you who may not know, the “W” stands for “white”, and is simply the color of the untreated, uncoated material. If your method references USP S1A, or Chromosorb-W/AW ...


    By Restek Corporation

  • Tips to maintain your HPLC & UHPLC systems and columns

    Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your ...


    By Waters Corporation

  • Glyphosate and AMPA in drinking water

    Carcinogenic or not? The broad-spectrum herbicide glyphosate is used all over the world in agriculture. Alongside farming, the chemical is also used for weed-killing in domestic gardens and in public and private spaces kept free from «vegetal invasion», such as railway tracks. Glyphosate has been used since the 1970s in pesticides and was hitherto thought to be harmless at ...


    By Metrohm AG

  • Comparison of simple ozonation and direct hydrogen peroxide processes in TNT removal from aqueous solution

    Oxidation of 2,4,6-trinitrotoluene (TNT) using simple ozonation and direct hydrogen peroxide was studied. An analytical method based on the HPLC (high performance liquid chromatography) system was used for TNT detection. The influence of parameters such as pH of the solution (3–10), initial TNT concentration (10–100 mg/L), dosage of ozone (0.2–1 g/hr), H2O2/TNT molar ratio (250–1,000), ...


    By IWA Publishing

  • What is the pressure limit for my LC column?

    This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ...


    By Restek Corporation

  • Is Microflow LC a Reality or Perception?

    Microflow or nanoflow chromatography has been used mostly in the academic and fundamental research environment in the past for its various advantages over analytical liquid chromatography, but rarely in the industrial settings. This is most likely because many of us believe it is a pain-in-the-neck technique to perform! But is this a reality or just a ...


    By Waters Corporation

  • The Advantages of a Targeted 2D LC Approach

    Webinar Highlights | Focus on the Relevant: The Advantages of a Targeted 2D Chromatography Approach Hi folks, I’m Daniel Root, Senior Product Marketing Manager for Multidimensional LC Systems at Waters. I recently conducted a webinar that addressed multidimensional liquid chromatography – an exceptional tool for the improvement of characterization and separation of ...


    By Waters Corporation

  • Method Scaling in Preparative HPLC: Analytical Method & Preparative Method

    VOC:  “I tried to scale up my analytical HPLC method to prep and it didn’t work.” Andy:  This is one of the most common things we hear from chemists wanting to do preparative chromatography. We also hear the following question pretty often too: VOC:  “I have a good analytical HPLC method, how do I scale it to ...


    By Waters Corporation

  • Impact of pH on Derivatization of Nitrophenol Compounds

    Our goal of evaluating the Normalized French Environmental Method 12673 required testing the method’s ability to derivatize nitrophenols and alkylphenols in addition to the chlorophenols the method was designed for. Unfortunately, nitrophenols proved challenging to derivatize at low concentrations. In an effort to improve the derivatization of nitrophenols, we experimented with the pH of ...


    By Restek Corporation

  • Enhanced separation of hop oils by flow-modulated GC×GC–TOF MS with Tandem Ionisation

    Beer contains hundreds of organic ingredients, with concentrations spanning many orders of magnitude, and including monoterpenes (C10) and sesquiterpenes (C15). These are aroma-active hydrocarbons found in the essential oils of various plants, including hops, and provide much of the characteristic ‘bitterness’ of the finished beer. Of the greatest importance for beer is the ...


    By SepSolve Analytical

  • Synthesis of granular nanozeolite NaA from Phragmites australis for removal of total petroleum hydrocarbon

    The purpose of this study was to synthesize granulated nanozeolite NaA particles from Phragmites australis in order to evaluate their efficacy for removal of total petroleum hydrocarbon (TPH). The perennial wetlands grass P. australis was chosen for study because it is one of the most abundant plants found growing around oil refineries. Fourier transform infrared ...


    By IWA Publishing

  • Out with the old, in with the new…

    We’re all familiar with the adage, “Out with the old, in with the new,” but we probably tend to think of it as it applies to clothes for donation or past relationships, and maybe not in terms of updating our old laboratory equipment. After attending my first WCBP conference, held in Washington, D.C. during January, and hearing life cycle management and ICH Q12 being discussed ...


    By Waters Corporation

  • Matrix LIMS configured and implemented within 3 months case study

    Laboratory staff at Glatfelter have established an extremely close, ongoing working relationship with Autoscribe. This has allowed the design and implementation in just 3 months of a configured Matrix Gemini LIMS for the company's corporate analytical services group. Organisation Established since 1864, global paper supplier Glatfelter has expanded its product range from high-quality ...


    By Autoscribe Informatics

  • ACQUITY Arc System: Transferring Validated HPLC Methods within USP Guidelines

    The thought of transferring established high performance liquid chromatography (HPLC) methods onto a another vendor’s LC, or a new platform like UHPLC/UPLC, may cause a bit of anxiety for those that may be unaware of how easy it can be. The reason that the topic of analytical method transfer induces so much angst is that for the transfer of an LC method to succeed, the method has to work as ...


    By Waters Corporation

  • An in vitro method for determining the bioaccessibility of pharmaceuticals in wildlife

    Wildlife can be exposed to human pharmaceuticals via prey that have accumulated the compounds from wastewater, surface water, sediment and soil. One factor affecting internal absorption of pharmaceuticals is bioaccessibility, the proportion of the compound that enters solution in the gastrointestinal tract. Currently, the bioaccessibility of most pharmaceuticals in prey remains unknown for ...


    By John Wiley & Sons, Ltd.

  • Separation of quercetin from a Ginkgo Biloba mother tincture using the PrepChrom C-700

    The aim of this application note was to isolate a flavonol, quercetin, from a Ginkgo Biloba mother tincture. The prepurification of the mother tincture was performed on a reversed phase flash column by the means of dry loading. The target compound was identified by its characteristic UV-VIS spectra and the retention time determined by running a standard. Subsequently, the purification of the ...


    By BÜCHI Labortechnik AG

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