HPLC Articles

  • Improved Productivity and Savings with RediSep Rf Gold® Columns

    Abstract The RediSep Rf Gold® high performance columns now provide organic chemists with more resolving capability in their flash chromatography purification through the utilization of smaller, spherical media. Finer particle sizes (20–40 µm) easily purify compounds that were previously unresolved with classic flash grade silica (40–60 µm, 60Å). RediSep ...


    By Teledyne ISCO

  • Enhanced Purification of Capsaicins with the CombiFlash® EZ Prep

    Abstract In this application note, capsaicins are used as a model for compounds difficult to resolve with flash chromatography. Capsaicin compounds possess very similar structures making them difficult to resolve from each other. Although these compounds can be resolved on flash chromatography columns, such as the RediSep® Rf Gold C18, preparative high pressure liquid chromatography ...


    By Teledyne ISCO

  • Higher Resolution Results with RediSep Rf Gold® Silica Columns

    Abstract The RediSep Rf Gold silica columns now provide organic chemists with more resolution capability in their Flash chromatography purification. Through smaller particle sizes, compounds that were previously unresolved with classic Flash grade silica (40–60 µm, 60Å) are now easily purified. RediSep Rf Gold silica columns are distinguished in the market by an ...


    By Teledyne ISCO

  • Loading capacity of silica flash cartridges

    FlashPure Cartridges Optimizing flash chromatography requires finding the ideal cartridge and conditions for a given sample. As the goal is to prepare the largest possible amount of pure sample in the shortest possible time and with a minimum use of solvent, it is crucial to know how high loadings affect the separation for different cartridges. Unlike analytical chromatography, preparative ...


    By BÜCHI Labortechnik AG

  • Waters HPLC & UHPLC Application Notes at Your Finger Tips

    Summary A quick reference notebook to Waters HPLC/UHPLC application note highlights.   140 application notes covering a wide range of markets, such as Pharma, Food, and Health Science. Condensed one page format highlighting experimental conditions and chromatographic results. Tips on how to choose the best column to deliver ...


    By Waters Corporation

  • Microfiltration: Rocker VF12 Kit Saves Time and Money

    Filtering organic solvents for applications such as HPLC buffer prep can be challenging in order to ensure chemical compatibility with filters (PVDF, Nylon, or PTFE), preserving longevity of an HPLC ...


    By Sterlitech Corporation

  • Pentachlorophenol degradation by Pseudomonas fluorescens

    Fluorescent Pseudomonads strains were considered as plant growth promoting bacteria. They exhibited antagonistic activities against phytopathogens and showed bio-fertilizing properties. The strain Pseudomonas fluorescens PsWw128, isolated from wastewater, can use the pentachlorophenol (PCP) as the sole source of carbon and energy. High-performance liquid chromatography (HPLC) and ...


    By IWA Publishing

  • The Clog Blog

    Clogging and over pressuring are definitely problematic for Liquid Chromatography columns. Back in 2005 John Dolan’s sage advice to chromatographers to make an LC column last forever was simple, “Never open the box!” As John pointed out, this is not practical and I have to agree. But as an LC column manufacturer I have seen remarks from customers regarding clogging as though it ...


    By Restek Corporation

  • Top 16 for 2016 - Restek LC article makes the cut

    If you are an avid chromatographer you likely subscribe to LC/GC magazine or its popular electronic version, E-Separation Solutions. It is definitely the place to go for news and information related to all things Chromatography. Over the years Restek has had many an article published in LC/GC and, full disclosure, we advertise there as well. Who in the business doesn’t? It is the place ...


    By Restek Corporation

  • Fluorophenyl LC Phases- what you should know

     They may be called PFP, PFPP, pentafluorophenyl, fluorophenyl or F5 phases. What they all have in common is the structure shown above (the pentafluorophenyl  group) attached to the silica particles. Most modern phases contain a propyl spacer between the above group and the silica, so that the phase looks something like this: ...


    By Restek Corporation

  • Separation of citral isomers from lemongrass oil

    Reveleris® Prep: Purification with flash chromatography followed by a prep-HPLC isolation of citral isomers Lemongrass is cultivated around the world. It's mainly grown for its oils, which have a number of uses including for vitamin A, perfumes, insect spray, cosmetics, perfumes, food and drinks. Lemongrass is also enjoyed as a tea throughout the world. Lemongrass oil contains several ...


    By BÜCHI Labortechnik AG

  • Why Biphenyl should be your first choice in LC.. A perspective from a “ GC” specialist..

    Being in GC for 38 years it made me wonder why in LC mostly C18 columns are used for developing a method. I never understood this as its against the basic rules for selecting the optimal stationary phase. In LC we see that about 95% of the applications is done using reversed phase materials, especially the C18 type. There are many C18 types available, and they all show a different separation ...


    By Restek Corporation

  • What can I use to filter my mobile phase?

    If you are using mobile phase that contains salts or, generally, any components that are weighed out for its preparation, you will need to filter the solution before using with HPLC or UHPLC. The most common type of filtration is membrane microfiltration. We sell an extensive group of products for this and they are discussed very well in a blog ...


    By Restek Corporation

  • Development of a Cleaning Validation Method for MICRO® A07 Citric Acid Cleaner Using HPLC-CAD

    Abstract A high performance liquid chromatography (HPLC) method with a Corona Charged Aerosol detector for the cleaning validation of MICRO* A07 Citric Acid Cleaner residue has been developed. The pooled average repeatability of 3 replicates at 6 concentration levels expressed as RSD was 1.3 % while the pooled average intermediate precision (intra-day repeatability in 3 days) of 3 replicates ...

  • A brief introduction to DiatoSorb-W

    DiatoSorb-W is a diatomaceous earth material that Restek will sell in bulk and in packed columns, uncoated and coated (with a liquid phase of your choice), which will replace Chromosorb-W packings. For those of you who may not know, the “W” stands for “white”, and is simply the color of the untreated, uncoated material. If your method references USP S1A, or Chromosorb-W/AW ...


    By Restek Corporation

  • Tips to maintain your HPLC & UHPLC systems and columns

    Recently, Ade Kujore (Cecil Instruments), Tracie Brombos (GenTech Scientific) and I were asked by Angelo De Palma, a well-respected writer for Lab Manager magazine, to contribute to an article about maintaining and caring about your laboratory instruments. Here are my tips/best practices for column and HPLC/UHPC/UPLC system maintenance. Maintaining your ...


    By Waters Corporation

  • Comparison of simple ozonation and direct hydrogen peroxide processes in TNT removal from aqueous solution

    Oxidation of 2,4,6-trinitrotoluene (TNT) using simple ozonation and direct hydrogen peroxide was studied. An analytical method based on the HPLC (high performance liquid chromatography) system was used for TNT detection. The influence of parameters such as pH of the solution (3–10), initial TNT concentration (10–100 mg/L), dosage of ozone (0.2–1 g/hr), H2O2/TNT molar ratio (250–1,000), ...


    By IWA Publishing

  • Iridium nanoparticles with high catalytic activity in degradation of acid red-26: an oxidative approach

    Nanocatalysis using metal nanoparticles constitutes one of the emerging technologies for destructive oxidation of organics such as dyes. This paper deals with the degradation of acid red-26 (AR-26), an azo dye by hexacyanoferrate (abbreviated as HCF) (III) using iridium nanoparticles. UV-vis spectroscopy has been employed to obtain the details of the oxidative degradation of the selected dye. ...


    By IWA Publishing

  • Removal of perfluorooctanoic acid (PFOA) in groundwater by nanofiltration membrane

    Perfluorooctanoic acid (PFOA) is very persistent in the environment and resistant to typical degradation processes. PFOA has been widely used in surface-active agents and as an emulsifier in several products and can contaminate groundwater. Groundwater is considered as an important source of water; hence removal of PFOA contamination in groundwater is needed. This study aimed to examine the ...


    By IWA Publishing

  • What is the pressure limit for my LC column?

    This is a question we get in Tech Services fairly often.  Unfortunately, we are not able to provide a published limit for every column that we produce, but here are a few of them that we have specified: For 3 and 5 um fully porous particle ...


    By Restek Corporation

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